Journal: Cellular and Molecular Immunology
Article Title: Fatty acid desaturation by stearoyl-CoA desaturase-1 controls regulatory T cell differentiation and autoimmunity
doi: 10.1038/s41423-023-01011-2
Figure Lengend Snippet: A – E Liquid chromatography‒electrospray ionization tandem mass spectrometry (LC‒ESI‒MS/MS) analysis was performed to define the lipidome of wild-type (wt) and Scd1 −/− naïve T cells ( n = 2 samples). A Fatty acid composition of all lipid classes. B – E Log 2 fold change abundance of all lipid classes ( B ), fatty acyl moieties within lysophosphatidylcholine (LPC, C ) and phosphatidylcholine (PC, D ), and docosahexaenoic acid (DHA) within all lipid classes ( E ) is shown ( Scd1 −/− vs. wt). Only detectable fatty acyl moieties and nonesterified fatty acids and downstream metabolites are reported. F LC‒MS/MS analysis was performed to determine the abundance of nonesterified fatty acids and downstream metabolites in wt and Scd1 −/− naïve T cells and spleen tissue ( n = 3 samples). Log 2 fold change abundance of all fatty acids and downstream metabolites is shown ( Scd1 −/− vs. wt). G mRNA expression of Pnpla2 in wt and Scd1 −/− naïve T cells ( n = 11 samples). H ATGL protein levels in wt and Scd1 −/− naïve T cells ( n = 4 samples). The results are pooled from two ( A – E , H ), three ( F ), or seven ( G ) independent experiments. Data are represented as the mean ( A – F ) or as the mean ± SEM ( G ). * P < 0.05; ** P < 0.01, calculated with two-tailed unpaired Student’s t-test ( G , H )
Article Snippet: For analysis of ATGL abundance, the following antibody was used: ATGL (NB110-41536, 1:100; Novus, Belgium, Brugge).
Techniques: Mass Spectrometry, Expressing, Two Tailed Test